Quantitative recovery of nucleic acids from excised gel pieces by isotachophoresis.

method of fixing samples before antibody staining (6), and it would be interesting to assess the suitability of such samples for FISH. Quantitation of successful FISH in tissue sections is not straightforward because of sample-to-sample variation, nuclear truncation in sectioning and variations across the slide in both tissue density and section width. Accurate comparison of FISH sensitivity following microwave vs. protease treatment would require a much wider study and is not reported here. The speed and simplicity of microwaving combined with both FISH results that can be consistently scored and the low rate of sample failure make it our method of choice for prostatic tissue and should make it useful in interphase FISH on tissue sections in general.